Resources
Peptide Reconstitution Guide
Step-by-step instructions for reconstituting lyophilised research peptides and storing them correctly in in vitro laboratory settings.
Lyophilised (unopened)
2–8 °C short-term · −20 °C long-term
After reconstitution
2–8 °C · aliquot & freeze at −20 / −80 °C
Common diluent
Bacteriostatic water (0.9% benzyl alcohol)
Before you begin
- Allow refrigerated or frozen vials to reach room temperature.
- Inspect the seal and lyophilised cake for moisture or damage.
- Prepare bacteriostatic water or sterile water per your SOP.
- Use clean gloves and a low-particulate work surface.
Calculate diluent volume and expected concentration with our peptide calculator before opening the vial.
Calculate diluent volume
Final concentration equals vial mass divided by solvent volume. A 10 mg vial plus 2 mL solvent yields 5 mg/mL. Choose a volume that keeps your planned aliquot in a comfortable pipette range — typically 50–500 µL.
Plan concentration before opening the vial — use the peptide calculator linked below.
Add diluent correctly
Direct bacteriostatic water slowly down the inside wall of the vial — not onto the lyophilised powder. High-velocity streams cause foaming and mechanical stress. After adding the measured volume, gently swirl or roll the vial until fully dissolved. Avoid vigorous shaking unless your protocol requires it.
Label and verify
- Label with compound name, concentration, batch number, and date.
- Compare appearance with the batch Certificate of Analysis.
- Log the vial in your laboratory inventory system.
Storage after reconstitution
Most reconstituted research peptides are stored at 2–8 °C for short-term use. For longer timelines, aliquot into microcentrifuge tubes and freeze at −20 °C or −80 °C to limit freeze-thaw damage. Keep vials upright, protected from light, and away from repeated room-temperature exposure.
Lyophilised material not yet reconstituted is best kept at −20 °C for extended storage, or at 2–8 °C for near-term use.
Related resources
Calculator tools, handling FAQs, and extended reading for laboratory planning.
For in vitro laboratory research only. Not for human or veterinary use. Follow your institutional SOP and the supplier COA for compound-specific handling.