Research tools
Peptide Concentration & Dilution Calculator
Work out stock-solution concentration (mg/mL) and the aliquot volume for a target mass of lyophilised research peptide. In vitro laboratory use only.
Vial contents (mg)
Required aliquot
Solvent volume to add (mL)
Enter your required aliquot above for tailored suggestions, or pick a volume below.
How this tool works+
This free calculator helps researchers plan reconstitution, concentration, and sample size in the lab. Enter vial mass (mg), solvent volume (mL), and required aliquot (mcg or mg). It returns stock concentration in mg/mL and transfer volume in mL and microlitres.
Results are planning aids for in vitro work only.
Unit conversions+
- 1 mL = 1000 µL
- 1 mg = 1000 mcg
- 1 µL = 0.001 mL
- Concentration (mg/mL) = vial mass (mg) ÷ solvent volume (mL)
Worked examples+
10 mg vial, 250 mcg aliquot, 2 mL solvent
Stock concentration: 5 mg/mL.
Transfer 0.05 mL (50 µL).
5 mg vial, 500 mcg aliquot, 1 mL solvent
Stock concentration: 5 mg/mL.
Transfer 0.1 mL (100 µL).
Example peptide scenarios+
BPC-157 (5 mg)
Reconstitute with 2 mL solvent → 2.5 mg/mL
250 mcg aliquot → transfer 0.1 mL (100 µL)
TB-500 (5 mg)
Reconstitute with 2 mL solvent → 2.5 mg/mL
500 mcg aliquot → transfer 0.2 mL (200 µL)
GHK-Cu (50 mg)
Reconstitute with 5 mL solvent → 10 mg/mL
1 mg aliquot → transfer 0.1 mL (100 µL)
MOTS-c (10 mg)
Reconstitute with 2 mL solvent → 5 mg/mL
250 mcg aliquot → transfer 0.05 mL (50 µL)
Frequently asked questions+
How do I reconstitute?
Enter vial contents and solvent volume; the tool returns stock concentration. Solvent choice and handling follow the receiving laboratory’s SOP. For in vitro research only.
What units does the calculator use?
Mass in mg/mcg, volume in mL/µL.
Why that solvent volume?
It favours a transfer typically between 50 and 500 µL, which is comfortable on a pipette. Very small transfers are less accurate.
What are the optional syringe marks?
Optional. Some labs use a 100-mark/mL graduated syringe to measure the same mL/µL volume. Marks are not a dose.